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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.0.dev0

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

        You can report bugs, suggest improvements and find the source code for MultiQC on GitHub: https://github.com/ewels/MultiQC

        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2019-06-04, 12:06 based on data in: /home/assekagiri/accreditation/16s_rRNA/qc


        General Statistics

        Showing 232/232 rows and 4/5 columns.
        Sample Name% Dups% GCLengthM Seqs
        S100_R1
        73.3%
        55%
        249
        0.1
        S100_R2
        36.6%
        54%
        249
        0.1
        S101_R1
        65.6%
        51%
        248
        0.2
        S101_R2
        41.2%
        50%
        247
        0.2
        S102_R1
        74.3%
        51%
        245
        0.1
        S102_R2
        43.9%
        50%
        245
        0.1
        S103_R1
        82.6%
        51%
        248
        0.4
        S103_R2
        59.1%
        49%
        248
        0.4
        S104_R1
        82.9%
        53%
        246
        0.3
        S104_R2
        63.9%
        52%
        245
        0.3
        S105_R1
        74.7%
        53%
        241
        0.2
        S105_R2
        47.2%
        51%
        240
        0.2
        S106_R1
        84.1%
        53%
        251
        0.4
        S106_R2
        56.0%
        52%
        251
        0.4
        S107_R1
        76.5%
        52%
        222
        0.2
        S107_R2
        57.1%
        50%
        221
        0.2
        S108_R1
        81.1%
        53%
        247
        0.3
        S108_R2
        54.0%
        52%
        246
        0.3
        S109_R1
        81.0%
        52%
        248
        0.3
        S109_R2
        50.7%
        51%
        248
        0.3
        S111_R1
        87.1%
        52%
        243
        0.3
        S111_R2
        78.2%
        51%
        243
        0.3
        S112_R1
        66.2%
        54%
        248
        0.0
        S112_R2
        24.4%
        52%
        248
        0.0
        S114_R1
        79.9%
        50%
        248
        0.3
        S114_R2
        51.6%
        49%
        248
        0.3
        S115_R1
        71.3%
        49%
        238
        0.1
        S115_R2
        35.5%
        48%
        237
        0.1
        S116_R1
        77.0%
        51%
        249
        0.2
        S116_R2
        46.0%
        50%
        249
        0.2
        S117_R1
        81.3%
        54%
        250
        0.3
        S117_R2
        49.2%
        53%
        250
        0.3
        S118_R1
        53.5%
        50%
        168
        0.0
        S118_R2
        42.1%
        48%
        165
        0.0
        S119_R1
        84.9%
        52%
        244
        0.4
        S119_R2
        70.7%
        50%
        244
        0.4
        S11_R1
        71.1%
        52%
        234
        0.1
        S11_R2
        53.9%
        51%
        234
        0.1
        S120_R1
        89.7%
        52%
        247
        1.5
        S120_R2
        77.0%
        50%
        246
        1.5
        S121_R1
        74.0%
        54%
        237
        0.3
        S121_R2
        52.7%
        53%
        235
        0.3
        S122_R1
        82.2%
        53%
        245
        0.4
        S122_R2
        73.5%
        51%
        244
        0.4
        S123_R1
        75.9%
        53%
        246
        0.2
        S123_R2
        49.7%
        51%
        246
        0.2
        S124_R1
        71.5%
        51%
        245
        0.1
        S124_R2
        42.5%
        49%
        244
        0.1
        S126_R1
        79.5%
        53%
        246
        0.4
        S126_R2
        49.4%
        51%
        246
        0.4
        S127_R1
        83.6%
        52%
        249
        0.5
        S127_R2
        70.1%
        51%
        249
        0.5
        S128_R1
        86.2%
        53%
        244
        0.2
        S128_R2
        72.3%
        51%
        243
        0.2
        S129_R1
        75.1%
        52%
        233
        0.1
        S129_R2
        54.8%
        50%
        232
        0.1
        S130_R1
        79.0%
        53%
        248
        0.4
        S130_R2
        52.5%
        51%
        248
        0.4
        S131_R1
        83.6%
        52%
        243
        0.2
        S131_R2
        64.9%
        51%
        242
        0.2
        S132_R1
        64.1%
        52%
        233
        0.1
        S132_R2
        35.3%
        51%
        231
        0.1
        S134_R1
        75.1%
        52%
        223
        0.1
        S134_R2
        57.0%
        50%
        221
        0.1
        S135_R1
        78.4%
        49%
        249
        0.3
        S135_R2
        54.0%
        48%
        249
        0.3
        S137_R1
        67.5%
        52%
        239
        0.1
        S137_R2
        44.2%
        50%
        238
        0.1
        S138_R1
        71.7%
        52%
        240
        0.1
        S138_R2
        35.7%
        51%
        239
        0.1
        S139_R1
        81.1%
        49%
        248
        0.4
        S139_R2
        60.6%
        48%
        248
        0.4
        S140_R1
        62.8%
        52%
        218
        0.0
        S140_R2
        34.6%
        50%
        216
        0.0
        S141_R1
        77.3%
        53%
        244
        0.2
        S141_R2
        48.1%
        51%
        243
        0.2
        S142_R1
        60.9%
        52%
        244
        0.0
        S142_R2
        34.1%
        50%
        243
        0.0
        S143_R1
        81.5%
        50%
        247
        0.3
        S143_R2
        58.6%
        49%
        247
        0.3
        S145_R1
        62.5%
        52%
        244
        0.1
        S145_R2
        34.4%
        50%
        243
        0.1
        S14_R1
        85.1%
        52%
        246
        0.2
        S14_R2
        66.5%
        51%
        246
        0.2
        S15_R1
        76.0%
        52%
        245
        0.2
        S15_R2
        50.8%
        51%
        246
        0.2
        S16_R1
        61.8%
        52%
        240
        0.1
        S16_R2
        30.7%
        50%
        239
        0.1
        S17_R1
        82.1%
        54%
        250
        0.2
        S17_R2
        54.4%
        52%
        250
        0.2
        S18_R1
        68.2%
        52%
        229
        0.1
        S18_R2
        45.8%
        51%
        230
        0.1
        S1_R1
        72.1%
        52%
        234
        0.1
        S1_R2
        51.6%
        51%
        234
        0.1
        S21_R1
        57.7%
        52%
        248
        0.0
        S21_R2
        22.2%
        51%
        248
        0.0
        S24_R1
        79.4%
        52%
        234
        0.1
        S24_R2
        55.1%
        51%
        234
        0.1
        S25_R1
        75.7%
        52%
        243
        0.2
        S25_R2
        44.3%
        51%
        243
        0.2
        S26_R1
        80.0%
        52%
        238
        0.1
        S26_R2
        64.3%
        51%
        238
        0.1
        S28_R1
        73.8%
        52%
        243
        0.1
        S28_R2
        48.5%
        51%
        243
        0.1
        S29_R1
        70.3%
        52%
        242
        0.1
        S29_R2
        49.7%
        51%
        242
        0.1
        S2_R1
        78.0%
        52%
        247
        0.3
        S2_R2
        48.6%
        51%
        248
        0.3
        S31_R1
        77.9%
        51%
        250
        0.1
        S31_R2
        41.0%
        50%
        250
        0.1
        S33_R1
        63.4%
        52%
        239
        0.0
        S33_R2
        38.9%
        51%
        238
        0.0
        S34_R1
        84.4%
        51%
        250
        0.2
        S34_R2
        54.9%
        50%
        249
        0.2
        S35_R1
        74.4%
        52%
        248
        0.1
        S35_R2
        54.4%
        51%
        247
        0.1
        S37_R1
        82.6%
        54%
        250
        0.2
        S37_R2
        52.0%
        53%
        250
        0.2
        S38_R1
        77.1%
        52%
        231
        0.2
        S38_R2
        54.0%
        51%
        232
        0.2
        S39_R1
        72.7%
        52%
        241
        0.1
        S39_R2
        52.6%
        50%
        242
        0.1
        S3_R1
        72.9%
        52%
        238
        0.0
        S3_R2
        41.5%
        51%
        238
        0.0
        S40_R1
        68.6%
        52%
        237
        0.1
        S40_R2
        43.8%
        50%
        236
        0.1
        S42_R1
        82.5%
        53%
        250
        0.2
        S42_R2
        47.8%
        52%
        250
        0.2
        S43_R1
        72.8%
        52%
        247
        0.0
        S43_R2
        37.8%
        51%
        247
        0.0
        S44_R1
        72.0%
        52%
        234
        0.1
        S44_R2
        42.0%
        50%
        235
        0.1
        S46_R1
        65.9%
        53%
        231
        0.1
        S46_R2
        45.0%
        51%
        231
        0.1
        S47_R1
        79.4%
        52%
        248
        0.2
        S47_R2
        44.9%
        51%
        248
        0.2
        S48_R1
        72.0%
        52%
        240
        0.0
        S48_R2
        42.4%
        50%
        240
        0.0
        S4_R1
        81.0%
        52%
        248
        0.2
        S4_R2
        53.5%
        51%
        247
        0.2
        S50_R1
        75.9%
        52%
        236
        0.1
        S50_R2
        59.7%
        51%
        235
        0.1
        S51_R1
        75.4%
        52%
        243
        0.1
        S51_R2
        49.7%
        51%
        244
        0.1
        S52_R1
        72.3%
        52%
        240
        0.0
        S52_R2
        28.9%
        51%
        240
        0.0
        S53_R1
        85.0%
        52%
        237
        0.2
        S53_R2
        73.0%
        51%
        237
        0.2
        S54_R1
        78.6%
        51%
        250
        0.2
        S54_R2
        46.9%
        50%
        249
        0.2
        S55_R1
        77.7%
        53%
        243
        0.2
        S55_R2
        55.1%
        52%
        244
        0.2
        S56_R1
        77.9%
        52%
        233
        0.1
        S56_R2
        63.5%
        51%
        233
        0.1
        S57_R1
        81.9%
        55%
        237
        0.2
        S57_R2
        62.2%
        54%
        237
        0.2
        S58_R1
        72.8%
        53%
        249
        0.1
        S58_R2
        35.4%
        52%
        249
        0.1
        S59_R1
        82.1%
        52%
        250
        0.2
        S59_R2
        50.0%
        51%
        250
        0.2
        S60_R1
        75.8%
        52%
        248
        0.1
        S60_R2
        38.5%
        50%
        248
        0.1
        S61_R1
        81.9%
        53%
        248
        0.2
        S61_R2
        55.3%
        52%
        248
        0.2
        S62_R1
        82.4%
        53%
        248
        0.2
        S62_R2
        54.6%
        52%
        248
        0.2
        S63_R1
        78.7%
        52%
        219
        0.1
        S63_R2
        55.7%
        51%
        217
        0.1
        S64_R1
        74.6%
        51%
        237
        0.2
        S64_R2
        49.7%
        50%
        236
        0.2
        S65_R1
        81.9%
        54%
        246
        0.2
        S65_R2
        59.8%
        52%
        246
        0.2
        S66_R1
        56.8%
        51%
        205
        0.1
        S66_R2
        32.5%
        50%
        203
        0.1
        S67_R1
        83.7%
        52%
        242
        0.2
        S67_R2
        71.8%
        51%
        242
        0.2
        S68_R1
        65.3%
        52%
        246
        0.1
        S68_R2
        28.6%
        51%
        246
        0.1
        S69_R1
        76.3%
        50%
        246
        0.2
        S69_R2
        46.7%
        49%
        246
        0.2
        S71_R1
        69.9%
        52%
        244
        0.2
        S71_R2
        34.7%
        51%
        244
        0.2
        S72_R1
        57.2%
        53%
        236
        0.2
        S72_R2
        35.4%
        52%
        235
        0.2
        S74_R1
        75.4%
        53%
        218
        0.2
        S74_R2
        55.9%
        51%
        216
        0.2
        S75_R1
        75.0%
        53%
        238
        0.2
        S75_R2
        56.5%
        51%
        238
        0.2
        S77_R1
        72.7%
        52%
        233
        0.1
        S77_R2
        48.3%
        50%
        232
        0.1
        S78_R1
        73.1%
        52%
        245
        0.2
        S78_R2
        49.8%
        51%
        245
        0.2
        S79_R1
        66.2%
        51%
        239
        0.1
        S79_R2
        41.8%
        49%
        236
        0.1
        S80_R1
        75.9%
        50%
        147
        0.1
        S80_R2
        56.1%
        49%
        144
        0.1
        S81_R1
        50.6%
        51%
        193
        0.0
        S81_R2
        37.8%
        50%
        188
        0.0
        S82_R1
        79.9%
        53%
        243
        0.3
        S82_R2
        48.7%
        51%
        242
        0.3
        S83_R1
        82.4%
        52%
        246
        0.2
        S83_R2
        57.1%
        51%
        246
        0.2
        S84_R1
        84.6%
        52%
        244
        0.3
        S84_R2
        74.7%
        51%
        243
        0.3
        S85_R1
        75.5%
        54%
        245
        0.3
        S85_R2
        45.6%
        52%
        245
        0.3
        S86_R1
        60.3%
        51%
        216
        0.1
        S86_R2
        32.0%
        50%
        214
        0.1
        S88_R1
        66.9%
        52%
        209
        0.0
        S88_R2
        44.4%
        50%
        205
        0.0
        S89_R1
        69.3%
        52%
        244
        0.2
        S89_R2
        42.8%
        50%
        242
        0.2
        S8_R1
        79.6%
        52%
        227
        0.1
        S8_R2
        62.3%
        50%
        227
        0.1
        S90_R1
        67.9%
        52%
        226
        0.1
        S90_R2
        47.8%
        50%
        224
        0.1
        S92_R1
        66.7%
        52%
        223
        0.1
        S92_R2
        50.3%
        50%
        219
        0.1
        S93_R1
        78.3%
        52%
        237
        0.3
        S93_R2
        55.3%
        51%
        236
        0.3
        S94_R1
        73.3%
        53%
        178
        0.1
        S94_R2
        58.9%
        52%
        173
        0.1
        S95_R1
        70.0%
        52%
        179
        0.0
        S95_R2
        52.6%
        50%
        174
        0.0
        S96_R1
        80.3%
        52%
        248
        0.2
        S96_R2
        55.1%
        51%
        248
        0.2
        S97_R1
        87.2%
        52%
        245
        0.3
        S97_R2
        71.0%
        51%
        244
        0.3
        S98_R1
        84.6%
        53%
        247
        0.4
        S98_R2
        63.3%
        52%
        246
        0.4
        S9_R1
        86.9%
        53%
        248
        0.1
        S9_R2
        61.5%
        51%
        248
        0.1

        FastQC

        FastQC is a quality control tool for high throughput sequence data, written by Simon Andrews at the Babraham Institute in Cambridge.

        Sequence Quality Histograms

        The mean quality value across each base position in the read. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called. See the FastQC help.

        Click a heatmap row to see a line plot for that dataset.

        rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Per Base N Content

        The percentage of base calls at each position for which an N was called. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Sequence Duplication Levels

        The relative level of duplication found for every sequence. See the FastQC help.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Overrepresented sequences

        The total amount of overrepresented sequences found in each library. See the FastQC help for further information.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).


        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. See the FastQC help. Only samples with ≥ 0.1% adapter contamination are shown.

        Flat image plot. Toolbox functions such as highlighting / hiding samples will not work (see the docs).